Review



hsvd  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier
Bioz Manufacturer Symbol Thermo Fisher manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    Thermo Fisher hsvd
    Hsvd, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hsvd/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    hsvd - by Bioz Stars, 2026-06
    90/100 stars

    Images



    Similar Products

    pmd201t hsvd elvd  (TaKaRa)
    99
    TaKaRa pmd201t hsvd elvd
    Schematic representation of viroid dimerization and subsequent assembly in a binary vector. a Two different pairs of primers are used to generate distal Bsa I recognition sites (magenta) in such a way that compatible ends for assembly can be obtained. b In a single reaction of simultaneous restriction and ligation the two viroid monomers (blue) are ligated between them and to a binary vector with compatible cohesive ends (orange). c Specifically, the viroid dimer is inserted into an expression cassette that contains and a duplicated 35S, a constitutive promoter for plant expression and a PoPit terminator. Additionally, this binary vector has a T7 promoter for in vitro transcription. The dimeric viroid cDNA sequence replaces a lethal gene ccdB, thus guaranteeing an optimal efficiency of the reaction. d Detail of the generation of the receptor–vector starting from <t>pMD201t</t> construct)
    Pmd201t Hsvd Elvd, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pmd201t hsvd elvd/product/TaKaRa
    Average 99 stars, based on 1 article reviews
    pmd201t hsvd elvd - by Bioz Stars, 2026-06
    99/100 stars
    		  Buy from Supplier
    hsvd  (Shilpa Medicare)
    90
    Shilpa Medicare hsvd
    Schematic representation of viroid dimerization and subsequent assembly in a binary vector. a Two different pairs of primers are used to generate distal Bsa I recognition sites (magenta) in such a way that compatible ends for assembly can be obtained. b In a single reaction of simultaneous restriction and ligation the two viroid monomers (blue) are ligated between them and to a binary vector with compatible cohesive ends (orange). c Specifically, the viroid dimer is inserted into an expression cassette that contains and a duplicated 35S, a constitutive promoter for plant expression and a PoPit terminator. Additionally, this binary vector has a T7 promoter for in vitro transcription. The dimeric viroid cDNA sequence replaces a lethal gene ccdB, thus guaranteeing an optimal efficiency of the reaction. d Detail of the generation of the receptor–vector starting from <t>pMD201t</t> construct)
    Hsvd, supplied by Shilpa Medicare, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hsvd/product/Shilpa Medicare
    Average 90 stars, based on 1 article reviews
    hsvd - by Bioz Stars, 2026-06
    90/100 stars
    		  Buy from Supplier
    hsvd infection  (Shilpa Medicare)
    90
    Shilpa Medicare hsvd infection
    Schematic representation of viroid dimerization and subsequent assembly in a binary vector. a Two different pairs of primers are used to generate distal Bsa I recognition sites (magenta) in such a way that compatible ends for assembly can be obtained. b In a single reaction of simultaneous restriction and ligation the two viroid monomers (blue) are ligated between them and to a binary vector with compatible cohesive ends (orange). c Specifically, the viroid dimer is inserted into an expression cassette that contains and a duplicated 35S, a constitutive promoter for plant expression and a PoPit terminator. Additionally, this binary vector has a T7 promoter for in vitro transcription. The dimeric viroid cDNA sequence replaces a lethal gene ccdB, thus guaranteeing an optimal efficiency of the reaction. d Detail of the generation of the receptor–vector starting from <t>pMD201t</t> construct)
    Hsvd Infection, supplied by Shilpa Medicare, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hsvd infection/product/Shilpa Medicare
    Average 90 stars, based on 1 article reviews
    hsvd infection - by Bioz Stars, 2026-06
    90/100 stars
    		  Buy from Supplier
    hsvd isolates  (Shilpa Medicare)
    90
    Shilpa Medicare hsvd isolates
    Schematic representation of viroid dimerization and subsequent assembly in a binary vector. a Two different pairs of primers are used to generate distal Bsa I recognition sites (magenta) in such a way that compatible ends for assembly can be obtained. b In a single reaction of simultaneous restriction and ligation the two viroid monomers (blue) are ligated between them and to a binary vector with compatible cohesive ends (orange). c Specifically, the viroid dimer is inserted into an expression cassette that contains and a duplicated 35S, a constitutive promoter for plant expression and a PoPit terminator. Additionally, this binary vector has a T7 promoter for in vitro transcription. The dimeric viroid cDNA sequence replaces a lethal gene ccdB, thus guaranteeing an optimal efficiency of the reaction. d Detail of the generation of the receptor–vector starting from <t>pMD201t</t> construct)
    Hsvd Isolates, supplied by Shilpa Medicare, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hsvd isolates/product/Shilpa Medicare
    Average 90 stars, based on 1 article reviews
    hsvd isolates - by Bioz Stars, 2026-06
    90/100 stars
    		  Buy from Supplier
    hsvd  (Kirin Brewery Company)
    90
    Kirin Brewery Company hsvd
    Schematic representation of viroid dimerization and subsequent assembly in a binary vector. a Two different pairs of primers are used to generate distal Bsa I recognition sites (magenta) in such a way that compatible ends for assembly can be obtained. b In a single reaction of simultaneous restriction and ligation the two viroid monomers (blue) are ligated between them and to a binary vector with compatible cohesive ends (orange). c Specifically, the viroid dimer is inserted into an expression cassette that contains and a duplicated 35S, a constitutive promoter for plant expression and a PoPit terminator. Additionally, this binary vector has a T7 promoter for in vitro transcription. The dimeric viroid cDNA sequence replaces a lethal gene ccdB, thus guaranteeing an optimal efficiency of the reaction. d Detail of the generation of the receptor–vector starting from <t>pMD201t</t> construct)
    Hsvd, supplied by Kirin Brewery Company, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hsvd/product/Kirin Brewery Company
    Average 90 stars, based on 1 article reviews
    hsvd - by Bioz Stars, 2026-06
    90/100 stars
    		  Buy from Supplier
    hsvd bayesian 0  (Bruker Corporation)
    90
    Bruker Corporation hsvd bayesian 0
    Schematic representation of viroid dimerization and subsequent assembly in a binary vector. a Two different pairs of primers are used to generate distal Bsa I recognition sites (magenta) in such a way that compatible ends for assembly can be obtained. b In a single reaction of simultaneous restriction and ligation the two viroid monomers (blue) are ligated between them and to a binary vector with compatible cohesive ends (orange). c Specifically, the viroid dimer is inserted into an expression cassette that contains and a duplicated 35S, a constitutive promoter for plant expression and a PoPit terminator. Additionally, this binary vector has a T7 promoter for in vitro transcription. The dimeric viroid cDNA sequence replaces a lethal gene ccdB, thus guaranteeing an optimal efficiency of the reaction. d Detail of the generation of the receptor–vector starting from <t>pMD201t</t> construct)
    Hsvd Bayesian 0, supplied by Bruker Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hsvd bayesian 0/product/Bruker Corporation
    Average 90 stars, based on 1 article reviews
    hsvd bayesian 0 - by Bioz Stars, 2026-06
    90/100 stars
    		  Buy from Supplier
    hsvd  (Thermo Fisher)
    90
    Thermo Fisher hsvd
    Schematic representation of viroid dimerization and subsequent assembly in a binary vector. a Two different pairs of primers are used to generate distal Bsa I recognition sites (magenta) in such a way that compatible ends for assembly can be obtained. b In a single reaction of simultaneous restriction and ligation the two viroid monomers (blue) are ligated between them and to a binary vector with compatible cohesive ends (orange). c Specifically, the viroid dimer is inserted into an expression cassette that contains and a duplicated 35S, a constitutive promoter for plant expression and a PoPit terminator. Additionally, this binary vector has a T7 promoter for in vitro transcription. The dimeric viroid cDNA sequence replaces a lethal gene ccdB, thus guaranteeing an optimal efficiency of the reaction. d Detail of the generation of the receptor–vector starting from <t>pMD201t</t> construct)
    Hsvd, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hsvd/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    hsvd - by Bioz Stars, 2026-06
    90/100 stars
    		  Buy from Supplier
    hsvd  (Illumina Inc)
    90
    Illumina Inc hsvd
    Schematic representation of viroid dimerization and subsequent assembly in a binary vector. a Two different pairs of primers are used to generate distal Bsa I recognition sites (magenta) in such a way that compatible ends for assembly can be obtained. b In a single reaction of simultaneous restriction and ligation the two viroid monomers (blue) are ligated between them and to a binary vector with compatible cohesive ends (orange). c Specifically, the viroid dimer is inserted into an expression cassette that contains and a duplicated 35S, a constitutive promoter for plant expression and a PoPit terminator. Additionally, this binary vector has a T7 promoter for in vitro transcription. The dimeric viroid cDNA sequence replaces a lethal gene ccdB, thus guaranteeing an optimal efficiency of the reaction. d Detail of the generation of the receptor–vector starting from <t>pMD201t</t> construct)
    Hsvd, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hsvd/product/Illumina Inc
    Average 90 stars, based on 1 article reviews
    hsvd - by Bioz Stars, 2026-06
    90/100 stars
    		  Buy from Supplier
    hsvd algorithm 22  (MathWorks Inc)
    90
    MathWorks Inc hsvd algorithm 22
    Schematic representation of viroid dimerization and subsequent assembly in a binary vector. a Two different pairs of primers are used to generate distal Bsa I recognition sites (magenta) in such a way that compatible ends for assembly can be obtained. b In a single reaction of simultaneous restriction and ligation the two viroid monomers (blue) are ligated between them and to a binary vector with compatible cohesive ends (orange). c Specifically, the viroid dimer is inserted into an expression cassette that contains and a duplicated 35S, a constitutive promoter for plant expression and a PoPit terminator. Additionally, this binary vector has a T7 promoter for in vitro transcription. The dimeric viroid cDNA sequence replaces a lethal gene ccdB, thus guaranteeing an optimal efficiency of the reaction. d Detail of the generation of the receptor–vector starting from <t>pMD201t</t> construct)
    Hsvd Algorithm 22, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hsvd algorithm 22/product/MathWorks Inc
    Average 90 stars, based on 1 article reviews
    hsvd algorithm 22 - by Bioz Stars, 2026-06
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Schematic representation of viroid dimerization and subsequent assembly in a binary vector. a Two different pairs of primers are used to generate distal Bsa I recognition sites (magenta) in such a way that compatible ends for assembly can be obtained. b In a single reaction of simultaneous restriction and ligation the two viroid monomers (blue) are ligated between them and to a binary vector with compatible cohesive ends (orange). c Specifically, the viroid dimer is inserted into an expression cassette that contains and a duplicated 35S, a constitutive promoter for plant expression and a PoPit terminator. Additionally, this binary vector has a T7 promoter for in vitro transcription. The dimeric viroid cDNA sequence replaces a lethal gene ccdB, thus guaranteeing an optimal efficiency of the reaction. d Detail of the generation of the receptor–vector starting from pMD201t construct)

    Journal: Plant Methods

    Article Title: Highly efficient construction of infectious viroid-derived clones

    doi: 10.1186/s13007-019-0470-4

    Figure Lengend Snippet: Schematic representation of viroid dimerization and subsequent assembly in a binary vector. a Two different pairs of primers are used to generate distal Bsa I recognition sites (magenta) in such a way that compatible ends for assembly can be obtained. b In a single reaction of simultaneous restriction and ligation the two viroid monomers (blue) are ligated between them and to a binary vector with compatible cohesive ends (orange). c Specifically, the viroid dimer is inserted into an expression cassette that contains and a duplicated 35S, a constitutive promoter for plant expression and a PoPit terminator. Additionally, this binary vector has a T7 promoter for in vitro transcription. The dimeric viroid cDNA sequence replaces a lethal gene ccdB, thus guaranteeing an optimal efficiency of the reaction. d Detail of the generation of the receptor–vector starting from pMD201t construct)

    Article Snippet: Viroid transcripts were generated by transcription of 400 ng of linearized pMD201t HSVd/ELVd (Digested with Eco RI) with T7 RNA polymerase (Takara, Kusatsu, Japan) for 3 h according to manufacturer instructions.

    Techniques: Plasmid Preparation, Ligation, Expressing, In Vitro, Sequencing, Construct

    Workflow proposed to obtain infectious clones of a viroid. Viroid sequence can be amplified from infected tissue by RT-PCR or from a DNA source by PCR. If the viroid sequence does not contain a bsa I recognition site, the viroidal cDNA can be directly assembled into the binary vector (pMD201t), replacing a lethal gene, in a simultaneous bsa I restriction and ligation. Conversely, if there the viroid contains a bsa I recognition site, it can be cloned using another IIs enzyme, such as Bsm BI. Once digested and purified, the viroid cDNA is dimerized by ligation to a previously digested pMD201t. The dimeric viroidal cDNA cloned into pMD201t (pMD201t-viroid) can be used to generate the infectious RNA transcript in vitro, using T7 RNA polymerase onto a linearized plasmid (digested with Eco RI). Additionally, the pMD201t-viroid can be transformed into Agrobacterium tumefaciens for transient plant transformation and subsequent production of infective RNA in vivo

    Journal: Plant Methods

    Article Title: Highly efficient construction of infectious viroid-derived clones

    doi: 10.1186/s13007-019-0470-4

    Figure Lengend Snippet: Workflow proposed to obtain infectious clones of a viroid. Viroid sequence can be amplified from infected tissue by RT-PCR or from a DNA source by PCR. If the viroid sequence does not contain a bsa I recognition site, the viroidal cDNA can be directly assembled into the binary vector (pMD201t), replacing a lethal gene, in a simultaneous bsa I restriction and ligation. Conversely, if there the viroid contains a bsa I recognition site, it can be cloned using another IIs enzyme, such as Bsm BI. Once digested and purified, the viroid cDNA is dimerized by ligation to a previously digested pMD201t. The dimeric viroidal cDNA cloned into pMD201t (pMD201t-viroid) can be used to generate the infectious RNA transcript in vitro, using T7 RNA polymerase onto a linearized plasmid (digested with Eco RI). Additionally, the pMD201t-viroid can be transformed into Agrobacterium tumefaciens for transient plant transformation and subsequent production of infective RNA in vivo

    Article Snippet: Viroid transcripts were generated by transcription of 400 ng of linearized pMD201t HSVd/ELVd (Digested with Eco RI) with T7 RNA polymerase (Takara, Kusatsu, Japan) for 3 h according to manufacturer instructions.

    Techniques: Clone Assay, Sequencing, Amplification, Infection, Reverse Transcription Polymerase Chain Reaction, Plasmid Preparation, Ligation, Purification, In Vitro, Transformation Assay, In Vivo